There is enough amount of sheath fluid to perform machine start up and sample acquisition. Bd facsdiva software offers efficient data management tools to simplify data storage as well as improve organization of flow cytometer data. Helps eliminate manual data entry of results after acquisition and analysis. Along with builtin database and database utilities, bd facsdiva software provides automated data export in a variety of formats including xml, fcs, and even zip, for multiple files concurrently. Refer to the bd facsdiva software reference manual for details. If you are not familiar with the windows operating system, s ee the documentation provid ed with your computer.
Designed for the bd facscanto and the bd facscanto ii flow cytometers, the software handles setup, acquisition, analysis, and automated loading of samples. Exit bd facsdiva software and shut down the computer. These tools provide a graphical view of the entire experiment and allow the quick addition of labels, keywords, and acquisition attributes to a single tube, multiple tubes, specimens, or. At the heart of the bd lsr ii is the new digital acquisition, allowing for additional parameters to be detected, and yielding more information per cell. The instrument can simultaneously measure forward scatter, side scatter, and up to eight fluorescent parameters using spatially separated 405 nm. The bd facsdiva software reference manual assumes you have a working. In this guide, you will find a description of bd facsdiva software features specific to the hts option. Bd lsr ii online help topics are compiled from the following sources.
May 17, 20 bd biosciences has developed a protocol for enabling application settings on machines running the facsdiva v6 software, to create settings that allow for standardized consistent fluorescence intensity target values to be obtained across experiments run on different days or instruments. Expanding confidence and flexibility in todays integrated laboratory bd facsdiva software is a collection of rich tools for flow cytometer and application setup, data acquisition, and data analysis that help streamline flow cytometry workflows for todays busy laboratory. It provides an assortment of functions that allows users to run manual or batch analysis and includes applications for singlecell or index sorting for stem. Mar 01, 2015 the first, oddly enough, is bd s very own index sorting manual facsdiva software manual and may not even be installed on your computer or available for download from bd s website. You can use either software to perform instrument quality control. The bd facsdiva software reference manual assumes you have a working knowledge of basic microsoft windows operation.
Facscanto ii standard operation protocol basic operation. Firsttime users of bd facsdiva software should read. For the demonstration, we will assume this is a 4color flow cytometric analysis. If you are not familiar with the windows operating system, see the documentation provided with your. Facsaria ii laboratory equipment pdf manual download. Comox bc, bc canada, v8w 3w6 bd high throughput sampler option for bd lsr ii for instructions on creating a user name and password, refer to the bd. Biexponential scaling in the bd facsdiva software reference manual. Fcs express can load fcs files from the bd facsdiva multicolor flow cytometry.
Bd facscanto clinical software set up the cytometer with bd facscanto clinical software, even if you will optimize and run samples using bd facsdiva software. Wait 10 seconds, then open the bd facsdiva software and log on. Bd facsdiva software basic experiment guide please note, this guide is not intended to take the place of inperson training but is to be used as a reference as needed after completing training with unified flow core staff. Reduces errors due to manual transcriptions, improving laboratory. See using the worksheet grid tab on page 186 in the bd facsdiva software reference manual. Bd facsdiva software provides new features to help users integrate flow systems into new application areas, including index sorting for. Quadrant gate segments can now be offset or rotated on a pivot point. The bd facsdiva software quick start guide contains tutorials designed to familiarize you with bd facsdiva software.
For information on how to operate and maintain your flow cytometer, refer to the instrument users. This manual contains reference information about bd facscanto clinical software. All other cytometer and fluidics cart functions are controlled by bd facscanto clinical software and bd facsdiva software provided with the instrument. The bd facsdiva option was designed as an integrated hardware software platform and introduces a number of additional benefits not directly related to the digital nature of the system. It can be accessed from the bd facsdiva software help menu help literature reference manual, or by doubleclicking the shortcut on the desktop. Workflow overview the following figure shows the steps for daily workflow using bd facsdiva software. The instrument can simultaneously measure forward scatter, side scatter.
Learn to use bd facsdiva v6 software with our free 6. Inspect bluegreen tubing connected to the syringespumps on the front and back of the hts unit where air is usually seen. Facscanto ii standard operation protocol basic operation 1. Dec 30, 2017 this video will show you how to install bd facsdiva 8. Bd biosciences facsdiva software features organization. For instructions on running a complete fluidics shutdown, refer to the fluidics shutdown section in the bd. The bd facsdiva software reference manual assumes that you have a working knowledge of basic microsoft windows operation. Bd facsdiva software in order for it to be recognized by the computer. Using application settings to standardize flow cytometry. Lsr ii has been recently upgraded with a violet laser with 6 detectors and an additional detector off the red laser for alexa fluor 700. This manual describes how to use bd facsdiva software. Bd facscanto ii instructions for use manual pdf download. In bd facsdiva software, select hts prime, 23x to remove air bubbles caught in tubing. Bd facscanto clinical software can import worklists from bd facs sample prep assistant spa software v2.
Page 4 if this is part of a daily shutdown, perform these steps. Provides bidirectional lis connectivity for bd facs spa software, bd facscanto clinical software, and bd facsdiva, bd worklist manager, bd multiset, and bd hlab27 software. The markers act both as regions and statistical markers. Please contact us to schedule time on the instrument, set up a training appointment or to discuss a future project. Bd facsdiva software provides convenient and easy experiment layout tools to simplify creation of experiments. Bd biosciences digital flow cytometry software provides. Bd hts user guide the danafarber flow cytometry core.
For information on how to operate and maintain your flow cytometer, see your cytometer manual. Compensation controls in the bd facsdiva software reference manual. This guide contains instructions for using bd facsdiva software version 6. Please contact us to schedule time on the instrument, set up a training appointment or to discuss a. I only came upon this after our bd service engineer sent me a copy of it. Argus voyanta real estate software is designed to give you a complete picture of your portfolio, putting new insights at your fingertips and highlighting key risks, and getting you ahead of the competition. Bd facsdiva tm software is a collection of rich tools for flow cytometer and application setup, data acquisition, and data analysis that help streamline flow cytometry workflows for todays busy laboratory. Biexponential display the biexponential display feature has been enhanced as follows. Bd facsaria ii quick reference manual pdf download. Interference there are no known incompatibilities with bd facscanto clinical. If diva is already running, install hts device and select reinitialize from hts dropdown menu.
These tools provide a graphical view of the entire experiment and allow the quick addition of labels, keywords, and acquisition attributes to a single tube, multiple tubes, specimens, or the entire experiment. Biexponential display is enabled by default, but can be disabled in user preferences, allowing more events to be recorded per experiment. Bd facs canto ii flow cytometer quick user guide v. If your last name is shorter than 3 letters, your login will be your full first. Bd facsdiva provides a list of tools simplifying the creation of experiments. Trainees will be expected to complete all three components of the dhvi flow facility training program.
Follow the cytometer setup wizard to complete setup. Run bd facsdiva software by doubleclicking the software icon on the desktop fig. Bd biosciences has developed a protocol for enabling application settings on machines running the facsdiva v6 software, to create settings that allow for standardized consistent fluorescence intensity target values to be obtained across experiments run on different days or instruments. Bd facsdiva software is a flexible data acquisition and analysis package designed for bd facs brand digital flow cytometers. The bd facsdiva software reference manual assumes that you have a working. View and download bd facsaria ii quick reference manual online. The first, oddly enough, is bd s very own index sorting manual facsdiva software manual and may not even be installed on your computer or available for download from bd s website. The software uses flexible features to simplify acquisition, including experiment templates and automated compensation calculation. Tip highlights features or hints that can save time and prevent difficulties. Make sure the following steps are done before running your samples. Bd facslink lis interface solution with bd facsdiva software elearning course developed in collaboration with data innovations, a leading middleware provider experienced in clinical applications, this laboratory information system lis interface solution allows the direct transfer of information between bd software and an lis, reducing errors commonly caused by manual transcriptions. Automatic biexponential scaling values can be manually adjusted in the. Safety cover must be on for software to execute prime function.
In the login window, scroll down through the list of user names to locate yours. Select exit only, and click launch bd facsdiva software, enter your user name and password, and click ok. A stepbystep manual for generating highresolution graphs and histogram overlays of flow cytometry data obtained with facsdiva software. For information, see using biexponential scaling in the bd facsdiva software reference manual. Bd facsdiva software reference manual bd lsr ii users guide bd high throughput sampler users guide table 2 text and keyboard conventions convention use.
Move a new full sheath box cubitainer from under the flowjo. Computer workstation runs two software packages facscanto clinical software for automated immunophenotyping and bd facsdiva software for manual immunophenotyping of laboratory developed tests ldt. With histogram you can look at the distribution of the cell intensities very easily. Lsr ii and lsrfortessa are operated using the same software, facsdiva. Compensation of a 7 color panel on the bd lsr ii uc merced scif stem cell instrumentation foundry. Updated to include bd facsdiva software, version 5. See the bd facsdiva software reference manual and the bd cytometer setup and tracking application guide for more details. For the demonstration, we will assume this is a 4color flow cytometric analysis experiment. In this guide, you will find a description of bd facsdiva software features specific to the hts. Bd lsr ii, bd lsrfortessa, bd facscanto, and bd facscanto ii flow cytometers modified with the hts can acquire samples from plates or tubes. In the login window, scroll down through the list of user names to. Communication between hts unit and software will now be connected.
If you are not familiar with the windows operating system, refer to the documentation provided with your computer. Default templates are now provided for certain instrument functions. Optimizing with bd facsdiva software to optimize cytometer settings in bd facsdiva software. If you are an experienced user, try the updated tutorials to become familiar with new software features. See dragging gates into the population hierarchy on page 232 in the bd facsdiva software reference manual. Set instrument to run and open bd facsdiva software. If you are a new user, use the tutorials to get started with the software. The instrument can simultaneously measure forward scatter, side scatter, and up to eight fluorescent parameters using spatially separated 405 nm solid state, 488 nm solid. Bd facscanto clinical software contains modules for dedicated clinical applications with automatic gating algorithms, while bd facsdiva software is nonapplication specific. Bd facscanto ii flow cytometer quick reference guide. This video will show you how to install bd facsdiva 8.
Research software single cell multiomics systems reagents clinical reagents clinical diagnostic reagents clinical reagent systems and kits quality and process control research reagents antibodies and buffers. Login to computer using healthcare or lowa domain credentials healthcare id or hawkin. Bd lsr ii, bd facscanto, and bd facscanto ii flow cytometers modified with the hts can acquire samples from plates or tubes. Bd facsdiva software is for in vitro diagnostic ivd use when used with ivd reagents and instruments. The bd lsr ii is the first and only aircooled, fourlaser benchtop flow cytometer with the ability to acquire up to 18 colors. See headers and footers on page 100 in the bd facsdiva software reference manual. The basic 2dimensional dot plots and density plots are naturally supported. New feature to apply gate coordinates such as shape and size between worksheets to other tubes in an open experiment. Use gating tools and subsetting methods to define the populations of interest. Bd facsdiva software quick reference guide for the bd lsr ii. The bd facsdiva software reference manual includes instructions or descriptions for installation and setup, workspace components, acquisition controls, analysis tools, and data management. Each software package has its own reference manual, provided on the documentation cd. Sophie novault bd facs diva software research institut.
Refer to the information supplied by the manufacturer for applicationspecific limitations. In addition, a printed copy can be requested from bd biosciences. Bd facsdiva software reference manual for version 8. Waste tank is empty and refill the bottom layer of waste tank with bleach. Bd facsdiva software provides a graphic of the cytometers physical optical configuration, showing the layout of detectors, filters, and mirrors. Compensation of a 7 color panel on the bd lsr ii youtube. This video describes the process of manual and auto compensation in bd facsdiva on the lsr ii. Cytometer can be placed in standby when not running plates, primes, cleans, etc. Download a free 30 day trial today to quickly analyze your diva data in fcs express. Cytometer function is controlled by bd facsdiva software. Power panel figure a5 facsdiva icon figure a4 control panel figure a2 sheath filter figure a3 roller clamp.
A stepbystep manual for generating highresolution graphs and histogram overlays of flow cytometry data obtained with facsdiva software supplementary material. Before starting your daily workflow, ensure that your labs software administrator has performed all the necessary tasks. To log in to the facsdiva software you need to locate the icon on the desktop and double click it. Other software fcs and setup files created within bd facscanto clinical software can be imported into bd facsdiva software v5.
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